Campylobacter News

National Symposium on Campylobacter Intervention

National Symposium on Campylobacter Intervention
December 3, 2017, Chicago Marriott, Downtown Magnificent Mile
Funded by USDA National Institute of Food and Agriculture (NIFA), a national symposium on intervention of Campylobacter in poultry will be held on December 3, 2017 in Chicago, in conjunction with the 98th annual Conference of Research Workers in Animal Diseases (http://crwad.org/). The symposium will provide the newest information on innovative strategies to control Campylobacter in the food chain of poultry production.  Feature presentations will be given by national and international experts from academia, industry, and government agencies. The symposium is suitable for researchers, graduate students, extension agents, food safety specialists, public health professionals, and regulatory officials. It is open to public and is tentatively scheduled from 9:00 am to 3:15 pm on Dec. 3, 2017. Attendance is free. Specific questions about the symposium can be directed to Dr. Orhan Sahin at Iowa State University (osahin@iastate.edu).
Preliminary Program
·   9:00 – 9:10 AM:  Welcome, introduction, and opening remarks. Qijing Zhang, Iowa State University
·   9:10 – 9:30 AM: NIFA’S Food Safety Programs and Impacts.  Max Teplitski, National Program Leader in Food Safety and Microbiology, USDA NIFA.
·   9:30 – 9:55 AM: Epidemiology and risk factors of Campylobacter in commercial broiler production. Orhan Sahin, Iowa State University
·   9:55 – 10:20 AM Industry perspective on Campylobacter control. Bruce Stewart-Brown, Perdue Farms.
·   10:20 – 10:45 AMCampylobacter jejuni glycoconjugate vaccines for poultry. Christine Szymanski, University of Georgia.
·   10:45 – 11:00 AM: Coffee Break
·   11:00 – 11:25 AM: Alternative approaches for Campylobacter control in poultry, Gireesh Rajashekara, The Ohio State University
·   11:25 – 11:50 AM: Challenges for Campylobacter control in poultry processing. Manpreet Singh, University of Georgia
·   11:50 – 1:30 PM: Lunch break
·   1:30 – 1:55 PM: Post-harvest control of Campylobacter in poultry, Catherine Logue, Iowa State University
·   1:55 – 2:20 PM: European perspective on Campylobacter control , Jaap Wagenaar, University of Utrecht, The Netherlands
·   2:20 – 2:45 PM: Preharvest control of Campylobacter in poultry, Jun Lin, University of Tennessee
·   2:45 – 3:10 PM: Regulatory perspectives on Campylobacter control in poultry, Stephanie Hretz, USDA FSIS.
·   3:10 – 3:15 PM: Closing remarks.  Qijing Zhang, Iowa State University
·   3:30 – 4:30 PM Advisory board meeting and panel discussion (Invitation only)

Gut microbiota composition influences the outcome of Campylobacter jejuni colonization in chickens

A study was conducted to evaluate the influence of the intestinal microbiota on C. jejuni colonization and local host immune response in chickens under laboratory conditions. Birds were treated with an antibiotic cocktail (broilers) or raised under germ-free (SPF layers) conditions. Following oral inoculation with C. jejuni at 18-days of age, colonization levels in the ceca, liver and spleen, and the local immune responses in the gut were compared between different groups. The results showed the birds with a conventional gut microbiota were significantly less susceptible to Campylobacter colonization in the ceca, spleen and liver compared with the birds with an altered gut microbiota. On the contrary, the local gut immune responses were substantially more prominent in birds with the altered microbiota composition. In addition, C. jejuni induced histopathological intestinal lesions only in the microbiota-altered birds. These results show that the gut microbiota composition significantly affect the colonization (and pathology) of chickens by C. jejuni both locally within the ceca as well as systemically in the spleen and liver. Once identified, key members of the microbiota responsible for this observation could be used as potential prebiotics for control of Campylobacter in poultry.

Different detection methodologies could have significantly different recovery rates for Campylobacter from broiler ceca

Currently, there is no gold standard test for detection of Campylobacter from chicken samples. The performance characteristics of the commonly used diverse panel of detection methodologies (direct culture, enrichment, and PCR) were determined for detection of Campylobacter from cecal contents of slaughter-aged commercial broiler flocks in the United Kingdom. Enrichment in adapted Exeter broth (lacking polymyxin B) with a resuscitation step (pre-incubation at 37˚C for 4 h) followed by subculturing on modified charcoal cefoperazone deoxycholate agar (mCCDA) was found the be the most sensitive (100%) method. Similarly, direct culture on mCCDA yielded very high recovery rate (~98%). On the other hand, enrichment methods using Preston broth and Bolton broth (following subculturing onto mCCDA) were significantly less sensitive. The real-time PCR was able to detect Campylobacter in only 80% of the samples. Interestingly, different enrichment methods promoted the recovery of different Campylobacter species (Exeter broth favoring C. jejuni, while Bolton broth supporting C. coli). These findings indicate that different detection methods could significantly influence the outcomes of studies on Campylobacter incidence in poultry and thus the impact on public health.

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